Kit and method for the capture of tumor cells

ABSTRACT

The invention relates to a kit and method for the capture of tumor cells in a body fluid sample. The kit and method of the invention can capture living tumor cells but not non-living tumor cells or cell fragments so that the tumor species can be further identified by further culture of the captured tumor cells. Also, the kit and method of the invention can readily identify whether a sample contains tumor cells and collect these tumor cells for further identification so that the presence of cancer and development of the metastasis and early relapse can be found.

FIELD OF THE INVENTION

The invention relates to a kit and method for the capture of tumor cellsin a body fluid sample or a serum-containing sample. Particularly, thekit and method of the invention are provided to capture circulatingtumor cells.

BACKGROUND OF THE INVENTION

Cancer is caused by cumulative multiple genetic mutations, which resultin the activation of oncogenes and/or the inactivation of tumorsuppressor genes. Cancer remains a major cause of mortality worldwide.Despite advancements in diagnosis and treatment, the overall survivalrate has not improved significantly in the past several years. Thereremains an unfulfilled need for more accurate detection and sensitivemeans of diagnosis of tumors.

Most cancer deaths are not caused by the primary tumor. Instead, deathresults from metastases, i.e., multiple widespread tumor coloniesestablished by malignant cells that detach themselves from the site ofthe original tumor and travel through the body, often to distant sites.If a primary tumor is detected early enough, surgery, radiation,chemotherapy, or some combination of these treatments can ofteneliminate it. Unfortunately, the metastatic colonies are harder todetect and eliminate and it is often impossible to treat all of themsuccessfully. Therefore, from a clinical point of view, metastasis canbe considered as the conclusive event in the natural progression ofcancer. Moreover, the ability to metastasize is the property thatuniquely characterizes a malignant tumor. Cancer metastasis comprisesthe following complex series of sequential events: 1. Extension from theprimary locus into surrounding tissues; 2. Penetration into bodycavities and vessels; 3. Release of tumor cells for transport throughthe circulatory system to distant sites; 4. Re-invasion of tissue at thesite of arrest; and 5. Adaptation to the new environment so as topromote tumor cell survival, vascularization, and tumor growth. Due tothe complexity of cancer and cancer metastasis, and frustrationresulting from the lack of effective treatment for late-stage cancerpatients, much effort has been invested in developing tests to detectdevelopment of metastasis and early relapse.

Circulating tumor cells (CTCs) are cancer cells that are shed fromeither the primary tumor or its metastases and that circulate in theperipheral blood. While metastases are directly responsible for themajority of cancer deaths, CTCs may constitute seeds for metastases andmay indicate the spread of the disease. The ability to identify CTCswhen they are very sparse (at most a few CTCs per ml) could allow earlydetection of indications of a cancer, or even of a precancerous growthbefore the appearance of evident clinical symptoms. Potential interestin detection of CTCs in peripheral blood was first suggested over acentury ago, but then subsided because they are difficult to detect byconventional methods due to low numbers of CTCs in a sample. Thechallenge is to develop an approach that is capable of both highlysensitive and highly specific identification and characterization ofrare tumor cells circulating in the blood, enabling such cells to bedistinguished from normal epithelial cells and leukocytes. Detection ofcirculating tumor cells could facilitate cancer prognosis, diagnosis ofminimal residual disease, assessment of tumor sensitivity to anticancerdrugs, and personalization of anticancer therapy. Highly sensitive andspecific identification of CTCs would also have potential application inearly diagnosis and screening of invasive cancers.

Molecular techniques based on PCR amplification of tumor-specificabnormalities in DNA or RNA have facilitated detection of occult(hidden) tumor cells. PCR-based tests capable of routinely detecting onetumor cell in one million normal cells have been devised foridentification of circulating tumor cells in various types ofcarcinomas. For example, Smith B. et al. develops reversetranscriptase-polymerase chain reaction to detect melanoma cells inperipheral blood (Lancet, 338: 1227-1236, 1991). However, these methodsmay not effectively distinguish viable tumor cells from normal cells.Since cell integrity is destroyed during DNA or RNA extraction, thisapproach precludes the analysis of cell morphology and phenotype, and somay be unable to distinguish material shed directly from normal tissueas opposed to from tumors, nor allow detection of several associatedchanges in the same cell.

Immunofluorescence microscopy enables analysis of cell morphology anddirect counting of identifiable presumptive tumor cells. Detection iscarried out by immunolabeling of cells using appropriate antibodies.However, there are so far no antibodies for tumor specific antigens usedto identify CTCs. In addition, immunomagnetic cell separation withimmunocytochemical labeling has been developed and evaluated fordetection of CTC clusters in colorectal carcinoma patients (ClinicalCancer Research, 2001, Vol. 7, pp.4080-4085). WO 2006050352 provides animproved cell adhesion matrix (“CAM”) and an improved cell isolationdevice for separating target cells such as tumor, fetal and angiogeniccells from blood or other fluid tissue samples. Furthermore, WO2009051734 discloses a device for capturing circulating, nonhemopoietictumor cells. The device includes a microfluidic channel to which isbound a tumor specific binding agent; and a pump producing a continuous,unidirectional shear stress of 0.1 to 20 dyn/cm² in the channel.However, the above techniques cannot effectively detect CTC.

It is apparent that there is need for a method and/or kit foridentifying cells in circulation having metastatic potential prior toestablishment of a secondary tumor, particularly during the early stagesof cancer.

SUMMARY OF THE INVENTION

One object of the invention is to provide a method for capture of tumorcells in a body fluid sample or a serum-containing sample, comprisingthe following steps:

(a) attaching endothelium-like or epithelium-like cells on a solidsupport,

(b) inducing an inflammatory reaction of the cells of (a) with one ormore inflammation-inducing agents;

(c) attaching white blood cells to the cells of (a); and

(d) adding a body fluid sample or a serum-containing sample to the solidsupport whereby the white blood cells of (c) and the endothelium-like orepithelium-like cells of (a) capture tumor cells contained in the bodyfluid sample or the serum-containing sample.

Another object of the invention is to provide a kit for capture of tumorcells in a body fluid sample or a serum-containing sample, comprising(a) a solid support; (b) endothelium-like or epithelium-like cells thatare attached on the solid support; (c) white blood cells that areattached to the cells of (b); and (d) one or more inflammation-inducingagents.

BRIEF DESCRIPTION OF THE DRAWING

FIG. 1 is directed to optical microscope photographs showing theattachment of tumor cells to the epithelium cells either with (B) orwithout (A) inducing an inflammation reaction on the epithelium cells byusing IL-1β. The “a” in the figure represents tumor cells (hepG2 cells)and the “b” represents epithelium cells.

FIG. 2 is directed to optical microscope photographs showing theattachment of tumor cells to the epithelium cells after inducing aninflammation reaction on the epithelium cells by using either IL-1β(FIG. 2.(A)) or TNF-alpha (FIG. 2(B)). The “a” in the figure representstumor cells (hepG2 cells) and the “b” represents epithelium cells.

FIG. 3 is directed to optical microscope photographs showing theattachment of tumor cells to the white blood cells (neutrophils) afterinducing an inflammation reaction on the epithelium cells by usingeither IL-1β (FIG. 3(B)) or TNF-alpha (FIG. 3(C)). FIG. 3(A) is acontrol group wherein no white blood cells were added. The “a” in thefigure represents tumor cells (hepG2 cells); the “b” representsepithelium cells; and the “c” represents white blood cells(neutrophils).

FIGS. 4(A) and 4(B) show photographs of whole blood samples containingthe living hep G2 cells in a dilution of 10 times and 1,000 times,respectively. FIG. 4(C) shows a photograph of the whole blood samplecontaining the living hep G2 cells without dilution and FIG. 4(D) showsa photograph of the whole blood sample containing the dead hep G2 cellsin a dilution of 1,000 times. The “a” in the figure represents tumorcells (hepG2 cells); the “b” represents epithelium cells; the “c”represents white blood cells (neutrophils); and the “d” represents redblood cells.

DETAILED DESCRIPTION OF THE INVENTION

This invention relates to a method and kit for capturing tumor cells ina body fluid sample or a serum-containing sample. The detection andconfirmation of tumor cells in the body fluid sample or theserum-containing sample can be achieved by utilizing the concept thatwhite blood cells can capture living, nucleus-containing heterogeniccells in a sample. The kit and method of the invention can captureliving tumor cells but not non-living tumor cells or cell fragments, sothat the tumor species can be further identified by further culture ofthe captured tumor cells. It is difficult to identify unknown tumorcells in a test sample because they are rare and have various size andepitope. The kit and method of the invention can readily identifywhether a sample contains tumor cells and collect these tumor cells forfurther identification so that the presence of cancer and development ofthe metastasis and early relapse can be identified.

In one aspect, the invention provides a method for capture of tumorcells in a body fluid sample or a serum-containing sample, comprisingthe following steps:

(a) attaching endothelium-like or epithelium-like cells on a solidsupport,

(b) inducing an inflammatory reaction of the cells of (a) with one ormore inflammation-inducing agents;

(c) attaching white blood cells to the cells of (a); and

(d) adding a body fluid sample to the solid support whereby the whiteblood cells of (c) and the endothelium-like or epithelium-like cells of(a) capture tumor cells contained in the body fluid sample or theserum-containing sample.

In one embodiment, the method can further comprise a step ofpre-treating the body fluid sample or serum-containing sample prior touse so that the sample is in a physiological condition. In anotherembodiment, the method can further comprise, a step of pre-treating abiological sample to form a body fluid sample or a serum-containingsample prior to use. In further another embodiment, the method can alsofurther comprise a step of harvesting the captured tumor cells afterstep (d).

In another aspect, the invention provides a kit for the capture of tumorcells in a body fluid sample or a serum-containing sample, comprising(a) a solid support, (b) endothelium-like or epithelium-like cells thatare attached on the solid support, (c) white blood cells that areattached to the cells of (b), and (d) one or more inflammation-inducingagents.

According to the invention, the “solid support” or “solid carrier” meansany solid phase material upon which the cells used in the capture of theinvention can be attached, ligated or immobilized. Solid supportencompasses terms such as “membrane,” “resin;” “solid phase,” “surface”and “support.” In one embodiment, the solid support is hydrophilic.Particularly, the surface of the solid support can be modified to behydrophilic. The process for the modification can be, but is not limitedto, oxygen plasma, water plasma and chemical treatment. This support maybe composed of organic polymers such as nitrocellulose membrane, nylonmembrane, polystyrene, polyethylene, polypropylene, polyfluoroethylene,polyethyleneoxy, and polyacrylamide, as well as co-polymers and graftsthereof. The solid support may also be inorganic, such as glass, silica,reverse-phase silica or a biochip. The configuration of the solidsupport may be in the form of beads, spheres, particles, granules, gel,or surface. Surfaces may be planar, substantially planar, or non-planar.The solid support may be configured in the form of a well, depression orother container, vessel, feature or location. Preferably, the solidsupport is a Petri dish or plate, preferably with a plurality of wellsin which an assay can be conducted. Preferably, the plate is amulti-well microtiter plate. In another embodiment, the surface of thesupport can have an electrode(s). The electrode(s) can detect thepresence of the captured tumor cells and their amount. Detection ofcells with electrodes is known in the art. U.S. Pat. No. 6,716,633provides a blood cell detector including an orifice section having asingle orifice, a first supplying section for supplying a first bloodspecimen into the orifice section, a second supplying section forsupplying a second blood specimen into the orifice section, and firstand second electrodes provided on opposite sides of the orifice fordetecting a change in impedance of each of the first and second bloodspecimens when the first and second blood specimens are selectivelycaused to pass through the orifice, and the reference is incorporatedherein by reference. In one embodiment, the electrode can be composed ofindium tin oxide (ITO), carbon nanotube, silicon, or titanium oxide. Theelectrode can produce dielectrophorestic force (DEP Force) by change ofelectric current frequency. The DEP force can attract or move cellsaway. The constant cells are captured in the area with the electrode andprove a quantitative counting area.

According to the invention, the “endothelium-like or epithelium-likecells” refer to endothelial cells or epithelium cells and theiranalogues. Preferably, the endothelium-like or epithelium-like cells areblood vessel cells, lymph vessel cells, oral epithelium cells or humanumbilical vein endothelial cells.

According to the invention, the “inflammation-inducing agent” refers toagents that can induce an inflammation reaction. Preferably, theinflammation-inducing agent is cytokin, growth factor, surface proteinor interleukin. Preferably, the inflammation-inducing agent is tumornecrosis factor or interleukin. More preferably, theinflammation-inducing agent is tumor necrosis factor-α, interleukin-1(IL-1), IL-2, IL-6, IL-8, IL-1β, interferon-gamma (IFN-gamma),interferon-alpha (IFN-alpha) or TNF-alpha.

According to the invention, “white blood cells” refer to cells of theimmune system defending the body against both infectious disease andforeign materials. In one embodiment, the white blood cells includeneutrophils, eosinophils, basophils, lymphocytes, monocytes andmacrophage. Preferably, the white blood cells are neutrophils andmacrophages. White blood cells migrate toward sites of inflammation andthey attach to foreign substances to kill them. According to theinvention, the white blood cells attach to living, nucleus-containingheterogenic cells. After an inflammation reaction is induced accordingto the invention, the white blood cells move to the endothelium-like orepithelium-like cells and attach thereto. In one embodiment of theinvention, the white blood cells used in the method of the invention mayoriginally exist in the body fluid sample. In another embodiment of theinvention, the white blood cells are added and applied in the invention.

According to the invention, the “body fluid sample” or “theserum-containing sample” may be derived from any biological source, suchas a physiological fluid, including whole blood; ascites; saliva, urine;synovial fluid; peritoneal fluid; amniotic fluid; cerebrospinal fluid;serosal fluid; spinal fluid and other constituents of the body which maycontain tumor cells. If the sample contains tumor cells, they can becaptured by the white blood cells when employing the kit and method ofthe invention because the tumor cells are nucleus-containing heterogeniccells that can be identified and recognized by white blood cells.According to one embodiment of the invention, the body fluid sample orthe serum-containing sample may be pre-treated prior to use by themethod and kit of the invention so that the sample is in a physiologicalcondition to facilitate capture of living tumor cells and use of livingcells in the method and kit of the invention. According to anotherembodiment of the invention, the method can further comprise a step ofpre-treating a biological sample to form a serum-containing sample.According to the invention, the biological sample is any kind of bodysample; preferably, the biological sample is bone marrow aspirates, bonemarrow homogenates, lymphoid tissue homogenates or tissue homogenates.Preferably, the biological sample is a solution containing tissue.Preferably, the sample is pre-diluted more than 10 times. In oneembodiment, the sample volume used in the invention ranges from about 5to about 30 mL. Preferably, the sample volume is from about 5 to about25 mL, about 5 to about 20 mL, about 5 to about 15 mL, about 5 to about10 mL, about 10 to about 25 mL, about 15 to about 25 mL, about 10 toabout 20 mL or 15 mL to 20 mL.

According to the invention, the tumor cells in the body fluid sample orthe serum-containing sample are derived or secreted from tumor tissues.Preferably, the tumor cells are circulating tumor cells. According tothe invention, after the tumor cells are captured, they can be furtherharvested by methods known in the art. For example, tumor cells or theirfragments are extensively washed in large spectrum antibiotics andantifungic solutions in completed DMEM (6-7 washes) to achieve anaseptic level compatible with standard cell culture conditions. Then,the cells or fragments are cut into small pieces of 1 to 2 mm³. Thesepieces are seeded in 6-well plates pre-coated with Po1yHEMA with 2 mL ofcomplete medium. Tumor fragments are cultivated for 2 d, and then themedium is retrieved and replaced by medium alone or drug-containingmedium for 24 h. The drug used is the topoisomerase-I inhibitormetabolite SN-38 (7 ethyl 10 hydroxycamptotecin), which is the activemetabolite of camptothecin at 10-5 mM/l, a concentration similar to thatobserved in tumors after i.v. administration of camptothecin in humans.At the end of this period, a washout is performed to eliminate the drug,and medium is added for 72 h.

The kit of the invention is used to practice the method of theinvention. According to the invention, the kit includes (a) a solidsupport, (b) endothelium-like or epithelium-like cells that are attachedto the solid support, (c) white blood cells that are attached to thecells of (b), and (d) one or more inflammation-inducing agent. Each ofthe above materials of (a), (b), (c) and (d) of the kit is placed in aseparate container such as a vial, tube, or the like. That is, each ofthe containers comprises one of the separate materials to be used in theassay. The kit may further include a set of instructions for using thekit to conduct the intended assay for capturing tumor cells in a bodyfluid sample.

In the kits and methods of the invention, the endothelium-like orepithelium-like cells attached to a solid support can be induced togenerate inflammation reaction. The inflammation reaction is induced byadding one or more inflammatory-inducing agents. After inflammation isinduced, white blood cells used in the kits and methods of the inventionwill migrate and attach to the inflamed cells. Since white blood cellswill capture living, nucleus-containing heterogenic cells, after a bodyfluid sample is added, the tumor cells in the sample will be captured bythe white blood cells. However, non-living cells, cell fragments andother contaminants in the sample will not be captured by the white bloodcells. By using the kit and method of the invention, living tumor cellsin a body fluid sample or a serum-containing sample can be separated forfurther identification using methods known in the art such as real timePCR or flowcytometry after culturing them (Barrett D L, Jensen R H, KingE B, Dean P N, Mayall B H. Flow cytometry of human gynecologic specimensusing log chromomycin A:, fluorescence and log 90° light scatter. JHistochem Cytochem 27(1):573-578, 1979; Darzynkiewicz Z: Acridine orangeas a molecular probe in studies of nucleic acids in situ. In: FlowCytometry and Sorting, Melamed M R, Mullaney P F, Mendelsohn M L (eds).John Wiley & Sons, New York, 1979, pp 285-316; Frost J K, Tyrer H W,Pressman N J, Albright C D, Vansickel M H, Gill G W: Automatic cellidentification and enrichment in lung cancer. I. Light scatter andfluorescence parameters. J Histochem Cytochem 27545-551, 1979; and FrostJ K, Tyrer H W, Pressman N J, Adams L A, Vansickel M H, Albright C D,Gill G W, Tiffany S M: Automatic cell identification and enrichment inlung cancer. In. Light scatter and two fluorescence parameters. JHistochem Cytochem 27557-559, 1979).

EXAMPLES Example 1 Attachment of Tumor Cells to Epithelium Cells

A glass plate with gelatin-modified surface was placed at the bottom ofa Petri dish and then about 50,000 human embryonic kidney 293T(HEK-293T) endothelial cells or human umbilical vein endothelial cells(HUVEC) were seeded on the glass plate. After adding DMEM and M199mediums to the Petri dishes, the cells were cultivated at 37° C., 5% CO₂for 24 hours. Subsequently, the DMEM and M199 mediums were removed fromthe Petri dishes and the Petri dishes were washed with PBS buffer.Subsequently, the fresh DMEM and M199 mediums were added to the Petridish. 50 ng of inflammation-inducing agents such as interleukin-1β(IL-1β) and tumor necrosis factor-alpha (TNF-α) were added to the Petridishes to induce inflammation reaction of HEK-293T and HUVEC for 24hours. The expression level of interleukin-6 (IL-6) was measured byEnzyme-linked immunoassay (ELISA). If the expression level of IL-6 ishigher than 30 ng/ml, an inflammation reaction occurs. Alternatively,the expression level of IL-6 can be detected by Flow Cytometry. The ICAMantibodies attached to fluorescein isothiocyanate (FITC) were added tothe Petri dishes to capture ICAM-1 (inter-cellular adhesion molecule 1)which was induced by IL-1 and TNFα and expressed by the endothelium. TheFITC fluorescein was measured by Flow Cytometry. If the expression levelof ICAM-1 is higher than 10 ng/ml, an inflammation reaction occurs.

The tumor cells, liver cancer cells hepG2, were transfected with greenfluorescence protein (GFP). The hepG2 cells with GFP were added to thePetri dishes. After shaking for 10 minutes, 15 ml PBS buffer was addedfor washing. After washing 3 times, the Petri dishes were examined usingoptical microscope and fluorescence microscopy, respectively.

FIG. 1 is directed to optical microscope photographs showing theattachment of tumor cells to the epithelium cells with (FIG. 1(B)) orwithout (FIG. 1(A)) inducing an inflammation reaction on the epitheliumcells by using IL-1β. In FIG. 1(A), after washing, no hepG2 cells wereattached to the epithelium cells, whereas in FIG. 1(B), after washing,it can be seen that hepG2 cells were attached to the epithelium cells.

FIG. 2 is directed to optical microscope photographs showing theattachment of tumor cells to the epithelium cells after inducing aninflammation reaction on the epithelium cells by using IL-1β (FIG.2.(A)) and TNF-alpha (FIG. 2(B)). In both FIGS. 2(A) and (B), afterwashing, it can be seen that hepG2 cells were attached to the epitheliumcells. After an inflammation reaction was induced by either IL-1β orTNF-alpha, the epithelium cells can capture the tumor cells.

Example 2 Attachment of Ttumor Cells to White Blood Cells (neutrophils)After Inducing an Inflammation Reaction on the Epithelium Cells

A glass plate with gelatin-modified surface was placed at the bottom ofa Petri dish and then about 50,000 human embryonic kidney 293T(HEK-293T) endothelial cells or human umbilical vein endothelial cells(HUVEC) were seeded on the glass plate. After adding DMEM and M199mediums to the Petri dishes, the cells were cultivated at 37° C., 5% CO₂for 24 hours. Subsequently, the DMEM and M199 mediums were removed fromthe Petri dishes and the Petri dishes were washed with PBS buffer.Subsequently, the fresh DMEM and M199 mediums were added to the Petridish. 50 ng of inflammation-inducing agents such as interleukin-1β(IL-1β) and tumor necrosis factor-alpha (TNF-α) were added to the Petridishes to induce inflammation reaction of HEK-293T and HUVEC for 24hours. The expression level of interleukin-6 (IL-6) was measured byEnzyme-linked immunoassay (ELISA). If the expression level of IL-6 ishigher than 30 ng/ml, an inflammation reaction occurs. Alternatively,the expression level of IL-6 can be detected by Flow Cytometry. The ICAMantibodies attached to fluorescein isothiocyanate (FITC) were added tothe Petri dishes to capture ICAM-1 (inter-cellular adhesion molecule 1)which was induced by IL-1 and TNFα and is expressed by the endothelium.The FITC fluorescein was measured by Flow Cytometry. If the expressionlevel of ICAM-1 is higher than 10 ng/ml, an inflammation reactionoccurs.

Neutrophils were added to the Petri dishes and they attached to theinflamed HEK-293T and HUVEC cells. The tumor cells, liver cancer cellshepG2, were transfected with green fluorescence protein (GFP). The hepG2cells with GFP were added to the Petri dishes. After shaking 10 minutes,15 ml PBS buffer was added for washing. After washing 3 times, the Petridishes were examined using optical microscope and fluorescencemicroscopy, respectively.

FIG. 3 is directed to optical microscope photographs showing theattachment of tumor cells to the white blood cells (neutrophils) afterinducing an inflammation reaction on the epithelium cells by using IL-1β(FIG. 3(B)) and TNF-alpha (FIG. 3(C)). FIG. 3(A) is a control groupwherein no white blood cells were added. In FIGS. 3(B) and (C), thehepG2 cells attached to the neutrophils and clustered together, whereasin FIG. 3(A) the hepG2 cells randomly distributed in the Petri dish.

Example 3 Capture of Tumor Cells in the Presence of Red Blood Cells

A glass plate with gelatin-modified surface was placed at the bottom ofa Petri dish and then about 50,000 human embryonic kidney 293T(HEK-293T) endothelial cells or human umbilical vein endothelial cells(HUVEC) were seeded on the glass plate. After adding DMEM and M199mediums to the Petri dishes, the cells were cultivated under 37° C., 5%CO₂ for 24 hours. Subsequently, the DMEM and M199 mediums were removedfrom the Petri dishes and the Petri dishes were washed with PBS buffer.Subsequently, the fresh DMEM and M199 mediums were added to the Petridish. 50 ng of inflammation-inducing agents such as interleukin-1β(IL-1β) and tumor necrosis factor-alpha (TNF-α) were added to the Petridishes to induce inflammation reaction of HEK-293T and HUVEC for 24hours. The expression level of interleukin-6 (IL-6) was measured byEnzyme-linked immunoassay (ELISA). If the expression level of IL-6 ishigher than 30 ng/ml, an inflammation reaction occurs. Alternatively,the expression level of IL-6 can be detected by Flow Cytometry. The ICAMantibodies attached to fluorescein isothiocyanate (FITC) were added tothe Petri dishes to capture ICAM-1 (inter-cellular adhesion molecule 1)which was induced by IL-1 and TNFα and is expressed by the endothelium.The FITC fluorescein was measured by Flow Cytometry. If the expressionlevel of ICAM-1 is higher than 10 ng/ml, an inflammation reactionoccurs.

Neutrophils were added to the Petri dishes and they attached to theinflamed HEK-293T and HUVEC cells. The question of whether the presenceof red blood cells affects the capture of tumor cells by white bloodcells was evaluated. The whole blood testing samples containing thehepG2 cells were collected. The blood samples were not diluted ordiluted 10 times and 1000 times, respectively. Then Wright's stain wasadded to the blood samples to label the hepG2 cells contained therein.After staining, the blood samples were added to the Petri dishes.Subsequently, the Petri dishes were examined using optical microscope.

In addition, the whole blood testing samples containing the hepG2 cellswere collected and frozen for 30 minutes. The blood samples were notdiluted or diluted 1000 times. Then Wright's stain was added to theblood samples to label the hepG2 cells contained therein. Afterstaining, the blood samples were added to the Petri dishes.Subsequently, the Petri dishes were examined using optical microscope.

FIG. 4 is directed to the optical microscope photographs showing thecapture of living or dead tumor cells in the whole blood samples withoutor with dilution 10 times or 1,000 times. FIGS. 4(A) and 4(B) show thephotographs of the whole blood samples containing the living hep G2cells in a dilution of 10 times and 1,000 times, respectively. FIG. 4(C)shows the photograph of the whole blood sample containing the livinghepG2 cells without dilution and FIG. 4(D) shows the photograph of thewhole blood sample containing the dead hepG2 cells in a dilution of1,000 times. This proves that the presence of red blood cells did notaffect the capture of the tumor cells by neutrophils.

What is claimed is:
 1. A method for the capture of circulating tumorcells in a body fluid sample or a serum-containing sample, comprisingthe following steps: (a) attaching endothelium or epithelium cells on asolid support, (b) inducing an inflammatory reaction of the cells of (a)with one or more inflammation-inducing agent, wherein theinflammation-inducing agent is tumor necrosis factor or interleukin; (c)adding white blood cells so that they attach on the cells of (a),wherein the white blood cells are neutrophilis, eosinophils, basophils,lymphocytes, monocytes or macrophages; and (d) adding a body fluidsample or a serum containing sample to the solid support whereby thewhite blood cells and the endothelium or epithelium cells capture tumorcells contained in the body fluid sample or the serum-containing sample,wherein the body fluid sample or the serum-containing sample ispre-diluted more than 10 times.
 2. The method of claim 1, which furthercomprise a step of pre-treating the body fluid sample or theserum-containing sample prior to use for the method so that the sampleis in a physiological condition.
 3. The method of claim 1, which furthercomprise prior to use for the method, a step of pre-treating abiological sample to a serum-containing sample.
 4. The method of claim3, wherein the biological sample is bone marrow aspirates, bone marrowhomogenates, lymphoid tissue homogenates or tissue homogenates
 5. Themethod of claim 1, which further comprises after step (d), a step ofharvesting the captured tumor cells.
 6. The method of claim 1, whereinthe solid support is hydrophilic.
 7. The method of claim 1, wherein thesolid support is composed of an organic polymer selected from the groupconsisting of nitrocellulose membrane, nylon membrane, polystyrene,polyethylene, polypropylene, polyfluoroethylene, polyethyleneoxy, andpolyacrylamide, as well as co-polymers and grafts thereof.
 8. The methodof claim 1, wherein the solid support is composed of inorganic materialsselected from the group consisting of glass, silica and reverse-phasesilica.
 9. The method of claim 1, wherein the configuration of a solidsupport is in the form of a bead, a sphere, a particle, a granule, agel, or a surface.
 10. The method of claim 1, wherein the solid supportis configured in the form of a well, a depression or a vessel.
 11. Themethod of claim 1, wherein the solid support is a Petri dish or amicrotiterplate.
 12. The method of claim 1, wherein the solid supportpossesses electrode(s) thereon.
 13. The method of claim 12, wherein theelectrode is composed of indium tin oxide (ITO), carbon nanotube,silicon, or titanium oxide.
 14. The method of claim 1, wherein theinflammation-inducing agent is tumor necrosis factor-α, interleukin-1(IL-1), IL-2, IL-6, IL-8, IL-1β, interferon-gamma (IFN-gamma),interferon-alpha (IFN-alpha) or TNF-alpha.
 15. The method of claim 1,wherein the white blood cells are neutrophils or macrophage.
 16. Themethod of claim 1, wherein the body fluid sample or the serum-containingsample is whole blood, ascites, saliva, urine, synovial fluid,peritoneal fluid, amniotic fluid, cerebrospinal fluid, serosal fluid orspinal fluid.
 17. The method of claim 1, wherein the sample volume ofthe body fluid sample ranges from about 5 to about 30 mL.
 18. The methodof claim 1, wherein the sample volume of the body fluid sample rangesfrom 5 to about 25 mL, about 5 to about 20 mL, about 5 to about 15 mL,about 5 to about 10 mL, about 10 to about 25 mL, about 15 to about 25mL, about 10 to about 20 mL or 15 mL to 20 mL.